HR Professionals Case Study Example | Topics and Well Written Essays - 3250 words

HR Professionals - Case Study Example as the report declares the primary need for J. & S Air Conditioning is to create a HR department. This department will help to provide a more dynamic picture of the actual nature of strategic management and stresses the need for HRM to be present at all levels of decision-making. Likewise, one which operates only at the administrative level will be seen to provide an incomplete service for the strategic business needs of the company. Although the model is developed from a national base, it is considered useful as a tool for analyzing the role of HRM in providing value-added in an international context. According to the report findings HR professionals do not have to fulfill all four roles themselves. Depending on the processes designed to reach the goal, the work may be shared by line managers, outside consultants, employees, technology, or other delivery mechanisms for HR activities. The tasks of the department will be to develop HR programs, implement thee programs, measure their success and introduce necessary changes in HRM. J. & S Air Conditioning can use traditional techniques of HR development but it should take into account strategic goals and tasks of the management. Recruitment and selection programs will help J. & S Air Conditioning to ensure adequate supply of staff and their performance. Effective recruitment and selection of employees for the core workforce that provides the organization with stability and continuity has become essential for organizational survival and presents another set of challenges. There is already intense competition for workers who are talente d enough to be a part of the core, and this is predicted to become even fiercer. Following Bohlander et al (2004) each member of the core is expected to perform multiple "jobs" and handle multiple responsibilities, as well as to maintain long-term loyalty to the organization. Thus, organizations need to attract and select workers who won't jump ship but who can swim from ship to shore (or ship to ship) as needed, and who can keep their bearings when pushed (Bohlander et al 2004). Online recruitment Online recruitment is one of the innovative solutions to effective and fast recruitment. One of the critical differences among Internet recruiters is the method by which their service matches candidates with jobs. So called bulletin boards, such as Monster Board and Career Mosaic have served primarily as on-line job posting and resume listing services whose main

Molecular Basis of Pernicious Anaemia Lab Report

Molecular Basis of Pernicious Anaemia - Lab Report Example This process is important because it is a requirement needed for the maturation of red blood cells, putting forward a relation with the disease2. Parietal cells are found in the gastric glands where there major function is to produce hydrochloric acid that is involved in the first stages of digestion. For gastric acid secretion, the gastric H positive or the proton pump enzyme that consists of ninety five kDa alpha subunit and a sixty to ninety kDa alpha subunit3. Auto anti bodies contained in serum from different patients having pernicious anaemia react with either or both of the alpha and beta subunits of the gastric proton pumps. Knowledge about this antibody response provides a useful diagnostic tool for the pernicious disease. Also, the most widely used method for analyzing protein mixtures is by gel electrophoresis. The most commonly used type of gel is the SDS polyacrylamide. It essential to realize that proteins are solubilized in sodium dodecyl-sulphate (SDS) , an anionic de tergent that binds to the hydrophobic regions of proteins causing them to unfold hence acquiring an overall negative charge. Inclusive is a reducing agent that is included to disrupt any disulfide bonds that are within the bonds. Avery good example of a commonly used reducing agent is the 2-Beta mercaptoethanol. On electrophoresing the proteins through a polyacrylamide gel, the negatively charged proteins migrate towards the anode and separation done with regard to their size, with smaller proteins moving the furthest. It is possible to perform an analysis of the size of proteins in the mixture if molecular weights of running proteins are known. This report tests antibody from the serum samples of different patients and the results to be used to identify if any of them reacts with gastric proton pump. The results acquired from the tests will hence provide a guide to finding a diagnosis of pernicious anaemia. For accurate results it is important to use two different methods which wil l determine the presence of anti-proton pump antibodies in serum samples4. Method one (Practical three) Separate proteins extracted from mouse stomachs by SDS poly acrylamide gel electrophoresis, and transfer the proteins to a nitrocellulose membrane. Stain a section of a mouse stomach with haemotoxylin and eosin so that it becomes familiar with the structure and cells types within the stomach. It is essential to realize that the cells in the mouse stomach are similar to those of a human’s anti-proton pump antibodies. For this reason, they cross react with the equivalent mouse proteins. Method two (practical 4) Perform Western Blotting on the gastric proteins using sera from patients who may or may not have pernicious anaemia. Western blotting has this procedure. First, an individual acquires the SDS-PAGE of mouse stomach proteins. These proteins are transferred to nitrocellulose membrane by electrophoresis. The membrane is stained with Ponceau S so as to confirm protein tran sfer from gel to membrane. After that process, the membrane is de-stained and then stored in blocking buffer until next usage. Use H19 to incubate strips of membrane and then wash the membrane strips and incubate in blocking buffer. The membranes are incubated in secondary anti-bodies and in luminous detection buffer. Lastly, the markers are reassembled and test strips are visualized with Chemi -doc system5. Also, perform immune peroxidase staining of mouse stomach